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HOW WE MAKE A DIFFERENCE

OUR MISSION


"Our work focuses on the use of nonlinear optical interactions between femtosecond-duration laser pulses and biological material as a tool for precise ablation of structures and quantitative observation of dynamical processes in live biological samples."


Multiple positions for post-doctoral researchers in the Schaffer-Nishimura Lab


icon_research   FEATURES

Our lab is interested in studying the contribution of multiple physiological systems to disease initiation and progression, with applications in neurodegenerative disease, cardiovascular disease, and cancer.  We would like to understand how the vascular, immune, inflammatory systems and cells native to a tissue interact in these diseases. A major challenge in such work is that model systems such as cell culture or even organotypic tissue culture cannot fully recapitulate all the different cell types involved in disease, so in vivo studies are required. However, it is experimentally difficult to study and manipulate cell-level dynamics in live animals. Recently, we have worked to develop technologies such as improved imaging using multiphoton microscopy that work in whole animals and have sufficient spatial and temporal resolution to quantify cellular dynamics. We also now have tools, femtosecond laser ablation, to produce targeted disruption with cellular-scale precision.

icon_news   NEWS

icon_paper   PUBLICATIONS

Aspirin treatment does not increase microhemorrhage size in young or aged mice (2019)

Neutrophil adhesion in brain capillaries reduces cortical blood flow and impairs memory function in Alzheimer’s disease mouse models (2019)

Deep convolutional neural networks for segmenting 3D in vivo multiphoton images of vasculature in Alzheimer disease mouse models (2019)

Surgical preparations, labeling strategies, and optical techniques for cell-resolved, in vivo imaging in the mouse spinal cord (2019)

Brain Capillary Networks Across Species: A few Simple Organizational Requirements Are Sufficient to Reproduce Both Structure and Function (2019)

Photo Credit: Dave Burbank, Kathryn Henion, or Schaffer-Nishimura lab members