U.S. Veterinary Immune Reagent Network

The use of large animals as intermediate models in immunological research has been hampered by the availability of immune reagents for many years. The US Veterinary Immune Reagent Network is collaborative project funded by the USDA to develop key reagents for immunological research in domestic animals. Reagents for five species (cattle, pig, horse, poultry, and fish) are developed by three core facilities. Then, the new reagents will be distributed to the species communities for further characterization and testing. More than 50 laboratories within the United States participate in this network. Our group at Cornell is one of the three core facilities producing recombinant proteins (immunoglobulins, TCRs, cytokine and chemokine receptors, and various CD molecules) for all species. We use a novel equine IgG fusion protein expression system that our laboratory has developed previously and that has already been used successfully to develop various recombinant proteins and monoclonal antibodies for immunological research in large animals.

Related publications:

  1. Wagner B, Burton A and Ainsworth DM. 2010. Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals. Vet. Res., 41: 47. VIEW ABSTRACT
  2. Burton AB, Wagner B, Erb HN and Ainsworth DM. 2009. Serum interleukin-6 (IL-6) and IL-10 concentrations in normal and septic neonatal foals. Vet. Immunol. Immunopathol., 132: 122-128. VIEW ABSTRACT
  3. Wagner B and Freer H. 2009. Development of a bead-based multiplex assay for simultaneous quantification of cytokines in horses. Vet Immunol Immunopathol. 127: 242-348. VIEW ABSTRACT
  4. Wagner B, Hillegas JM, Flaminio MJ, and Wattrang E. 2008. Monoclonal antibodies to equine interferon-alpha (IFN-alpha): new tools to neutralize IFN-activity and to detect secreted IFN-alpha. Vet Immunol Immunopathol. 125: 315-325. VIEW ABSTRACT
  5. Wagner B, Hillegas JM, Brinker DR, Horohov DW, and Antczak DF. 2008. Characterization of monoclonal antibodies to equine interleukin-10 and detection of T regulatory 1 cells in horses. Vet Immunol Immunopathol. 122: 57-64. VIEW ABSTRACT
  6. Wagner B, Hillegas JM, and Antczak DF. 2006. A monoclonal antibody to equine interleukin 4. Vet. Immunol. Immunopathol. 110: 363-367. VIEW ABSTRACT
  7. Wagner B, Robeson J, McCracken M, Wattrang E, and Antczak DF. 2005. Horse cytokine/IgG fusion proteins – mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines. Vet. Immunol. Immunopathol. 105: 1-14. VIEW ABSTRACT