Equine Reagents

The following table lists the monoclonal antibodies specific for equine cytokines, chemokines, immunoglobulins, and CD markers developed by Dr. Bettina Wagner’s laboratory at Cornell University.

To obtain monoclonal antibodies, please contact Dr. Bettina Wagner.
 

Group of
Molecules
Specificity
(anti-equine)
Clone Mouse Isotype Methods Tested Reference
Cytokines IL-2 158-1 IgG1 FACS, ELISA, western blot, bead-based assay Freer et al. 2017. Abstract
IL-4 25 IgG1 FACS, ELISA, bead-based multiplex assay Wagner et al. 2012. Abstract
13G7 IgG1 FACSELISA, bead-based multiplex assay Wagner et al. 2006. Abstract
IL10 165-1 IgG1 FACSELISA Wagner et al. 2007. Abstract
492-1 IgG2b FACS, ELISA, bead-based multiplex assay
492-2 IgG1 FACS, ELISA, bead-based multiplex assay
IL-17A 76 IgG1 FACS, ELISA Perkins et al. 2014. Abstract
79-3 IgG1 ELISA, bead-based multiplex assay Wagner et al. 2015. Abstract
80-1 IgG1 ELISA, bead-based multiplex assay
IFN-α 29B IgG1 Low neutralizing activity, ELISA Wagner et al. 2008. Abstract
240-2 IgG1 High neutralizing activity, ELISA, bead-based multiplex assay
150 IgG2b No neutralizing activity, bead-based multiplex assay
IFN-γ 3 IgG1 ELISA, bead-based assay Wagner et al. 2015. Abstract
36 IgG1 ELISA, bead-based multiplex assay
38-1 IgG1 FACS
Chemokines CCL2 49 IgG1 ELISA, bead-based multiplex assay Schnabel et al. Accepted
53 IgG1 FACS, ELISA
104-2 IgG1 ELISA, multiplex
CCL3 77 IgG1 ELISA, bead-based multiplex assay
289 IgG1 ELISA, bead-based multiplex assay
290 IgG1 FACS
CCL5 46 IgG1 ELISA, bead-based multiplex assay
91 IgG1 ELISA, bead-based multiplex assay
96 IgG1 FACS
CCL11 24 IgG1 FACS, ELISA, bead-based multiplex assay
25 IgG1 ELISA, bead-based multiplex assay
CXCL10 44-2 IgG1 FACS Schnabel et al. Submitted
Immunoglobulins IgM 1-22 IgG1 Western blot, ELISA, West Nile virus IgM capture Wagner et al. 2007. Abstract
2B-63 IgG1
IgG1/3 159 IgG1 ELISA, bead-based multiplex assay Keggan et al. 2013. Abstract
IgG3/5 586 IgG1 ELISA, bead-based multiplex assay
IgG5 416-2 IgG2a ELISA, bead-based multiplex assay
522 IgG1 ELISA
IgG6 125-1 IgG1 ELISA, bead-based multiplex assay
267 IgG1 ELISA, bead-based multiplex assay
IgE 134 IgG1 FACS, ELISA, western blot Wagner et al. 2003. Abstract
176 IgG1
IgA 84-1 IgG1 FACS, Western blot, bead-based assay Schnabel et al. 2017
. Abstract
161-1 IgG1
CD Marker CD14 105 IgG1 FACS, cell sorting, ELISA, bead-based assay Kabithe et al. 2010. Abstract
59 IgG2b Western blot, ELISA, bead-based assay Wagner et al. 2013. Abstract
CD23 51-3 IgG1 FACS Wagner et al. 2012. Abstract
54-3 IgG1 Western blot
63-2 IgG1
CD25 15-1 IgG1 FACS Not yet published
28-1 IgG1
54-2 IgA

mAbs under development

mAbs for the following equine immune targets are currently under development and can be obtained for confirmatory testing before they are added to the chart above. Note that the specificity of the mAbs below is not yet confirmed:


Targets not resulting in a mAb

The following targets were approached and dropped during our reagent development projects for the horse because they did not result in a specific mAb. A brief reason for development failure is given for each target. For more information please contact Dr. Bettina Wagner.


Antibody development was funded by USDA/NIFA grants as part of the US-VIRN project, the Equine Immune Reagent Development grant, the Morris Animal Foundation, and the Harry M. Zweig Memorial Fund for Equine Research at Cornell University.